Mobiele menu

High sensitivity mass spectrometer for identification and analysis of proteins and other bio-macromolecules

Projectomschrijving

Verslagen


Samenvatting van de aanvraag

Mass spectrometry of bio-macromolecules, in particular of proteins, is rapidly becoming a key technique in modern biomolecular and biomedical sciences. Due to its extreme sensitivity it allows investigators to unambiguously identify femtomolar amounts of proteins on the basis of the rapidly growing information in the international sequence databases. Furthermore, mass spectrometry is the only technique that allows rapid and precise identification and analysis of post translational modifications of proteins. These unique features make mass spectrometry a technique that will soon be indispensable for efficient molecular and cellular biology in general and biomedical research in particular. In the Amsterdam area many large research institutions are active in the field of biomedical sciences and intensively use biomolecular approaches. They will directly benefit from this protein-oriented mass spectrometry facility. To this end, the Netherlands Cancer Institute (NKI) the Amsterdam Medical Centre (AMC) and the research school BioCentrum Amsterdam of the University of Amsterdam (UvA) and the Vrije Universiteit (VU) have joined forces with the Institute of Mass Spectrometry at UvA. Highly sensitive mass spectrometry requires very specific expertise and an infrastructure that warrants optimal operation of this type of complex equipment and allows reliable interpretation of the data. Such expert environment is available at the Institute of Mass Spectrometry (scientific director prof.dr. N.M.M. Nibbering) at the Chemistry Faculty of the University of Amsterdam. This institute is among the world leaders in mass spectrometry. It has, initiated by a grant from SON for an electrospray mass spectrometer in 1994 to the E.C. Slater Instituut (part of BioCentrum Amsterdam), accumulated specific expertise in the field of mass spectrometry of proteins. The ECSI has expert knowledge in the field of protein chemistry and analysis, including microsequencing in combination with mass spectrometry. Together, the two institutes will develop new techniques for mass spectrometric analysis of proteins and implement applications and adaptations developed elsewhere. Furthermore, they will be responsible for the dissemination of these techniques in the biomedical and biomolecular community. Moreover, the Chemistry Faculty of the UvA is committed to create a chair in macro-molecular mass spectrometry. In this application we propose to invest in a state-of-the-art Q-TOF mass spectrometer, which will be 1.installed in the Institute for Mass Spectrometry, 2.run by the E.C. Slater Instituut and the Institute of Mass Spectrometry co-operatively, and 3.available to all investigators in the AMC, NKI and BioCentrum Amsterdam, as well as those elsewhere in the Netherlands. The total investment is 1.4 Mio Gld.

Onderwerpen

Kenmerken

Projectnummer:
90134146
Looptijd: 100%
Looptijd: 100 %
1998
2004
Onderdeel van programma:
Projectleider en penvoerder:
Prof. dr. R. Driel
Verantwoordelijke organisatie:
Universiteit van Amsterdam